Laboratory Resources
Stanley Manne Children's Research Institute offers essential and shared laboratory resources for faculty, staff, and students. This page will provide an overview of laboratory supplies, research safety information, and shared equipment to enhance research productivity throughout Manne Research Institute.
Laboratory Supplies
Lab Gases and Dry Ice: Dry/wet ice, laboratory gases, liquid nitrogen, and gas tank regulators and valves are available through Procurement and Payment Services at Northwestern University. Find Lab Gases Order Forms here.
For more information, email labservices@northwestern.edu or call 312.503.8828.
It is required that faculty, staff, postdocs, research associates, students, and visitors working in laboratories or laboratory support facilities using compressed or liquefied gases get appropriated training. Go to myHR Learn to complete your safety training.
Training includes:
- Compressed gas cylinders certification
- Filling and maintenance of liquid nitrogen tanks certification
Guide to Compressed or Liquefied Gases in Laboratories
Fisher Stockroom in Simpson Querrey Biomedical Research Center
There is a Fisher stockroom on the mezzanine level of Simpson Querrey Biomedical Research Center where researchers can purchase research supplies. Chart string information
Location: Simpson Querrey Mezzanine level Room #1M-212
Hours: 8:00 a.m.-4:30 p.m., closed for lunch from noon to 1:00 p.m.
Contact: Stephen Gage, 312.503.7520, NUChicagoSQ.Storeroom@thermofisher.com
Research Safety Information
Contact Research Safety at Northwestern University for required safety training and general laboratory safety, hazardous materials shipping, and hazardous waste.
For medical treatments, contact Research Safety at Northwestern University
Incident Report forms obtained from the Research Safety at Northwestern University
Personal Protective Equipment
White Laboratory Coats
The Office for Research Safety at Northwestern University provides white lab coats and lab coat laundry program. Researchers may drop off a dirty coat and take a clean one. Personalized coats are not accepted.
Drop-off and pick-up locations are in the Fisher stockroom in Simpson Querrey Mezzanine level Room #1M-212 or Searle B652.
Additional PPE such as safety glasses, face shield, and cryogenic gloves can be obtained through Research Safety at Northwestern University.
Helpful Contact Information
Research Safety Northwestern University 3-8300
Facilities maintenance (e.g., leaks, temperature) contact Northwestern Facilities 3-8000
Medical emergency call 911
Shared Equipment Available at Stanley Manne Children’s Research Institute
Equipment are available to researchers from Stanley Manne Children’s Research Institute. Trained Lurie Children's users have 24/7 access to the equipment and are given permission to schedule their own sessions.
We expect the guidelines to be followed by every user utilizing any shared equipment at all times. When using the shared equipment please sign in with the log sheets near the equipment. Please report any instances of non-compliance or concerns to Joann Taylor or Greg Taborn.
Location: 10-308
Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.
OCR and ECAR rates are key indicators of mitochondrial respiration, glycolysis, and ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
After receiving training from our personnel or Aggilent Representative, users will get access to the room and will be able to reserve the instruments and run their experiments independently.
Send your request for training via email to Greg Taborn and Joann Taylor.
Scheduling for Seahorse XFe96 Analyzer is on a first come, first serve basis. Notification of cancellations is also greatly appreciated.
Location: 4-318
The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. The Chromium Controller enables integrated analysis of single cells at massive scale.
If you are a first-time user requesting training on Chromium Controller 10x Genomics Single Cell please email Greg Taborn and Joann Taylor with an appointment request.
Location: 3-509
The CLARIOstar Plus is a multi-mode plate reader. It comes equipped with patented LVF Monochromators, filters, and spectrometer.
- UV/vis absorbance
- Fluorescence intensity, including FRET
- Luminescence (flash and glow), including BRET
- Fluorescence polarization/anisotropy
- Time-resolved fluorescence, including TR-FRET
Please email Greg Taborn and Joann Taylor, if you would like to use the CLARIOstar Plus.
Location: 10-510
The QuickPlex instrument offers access to high-performance electrochemiluminescence immunoassays.
Please email Greg Taborn and Joann Taylor, if you would like to use the 1300 MESO QuickPlex SQ120 Imaging System.
Locations: 4-307, 4-509 and 10-421
The QuantStudio 6 Flex real-time PCR systems can easily interchange between 96-well (0.1 and 0.2 mL) and 384-well formats. The systems can detect differences as small as 1.5-fold in singleplex reactions with 10 logs dynamic range.
Please email Greg Taborn and Joann Taylor, if you would like to use the QUANTSTUDIO 6 FLEX PCR system.
Locations: 4-330 and 3-509
The G:BOX Chemi XX9 gel doc system has highest resolution cameras for imaging multiple sample types and sizes, from fluorescence 1D and 2D gels to chemiluminescent blots
Please email Greg Taborn and Joann Taylor, if you would like to use the IMAGER CHEMILUMINESCENT G-BOX.
Location: 3-509
The Bioruptor uses a system to uniformly process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications.
Please email Greg Taborn and Joann Taylor, if you would like to use the Bioruptor Plus sonication device.
Location: 3-509
The Bioruptor Pico allows flexibility in sample volumes (20 µl - 2 ml) and a fast parallel processing of samples (up to 16 samples simultaneously). The built-in cooling system (Bioruptor Cooler) ensures high precision temperature control.
Please email Greg Taborn and Joann Taylor, if you would like to use the Bioruptor Pico sonication devive.
The 4D-Nucleofector X Unit supports Nucleofection of various cell numbers (2 x 104 to 2 x 107 ) in different formats.
Please email Greg Taborn and Joann Taylor, if you would like to use the 4D-Nucleofector X Unit.
The Y Unit of the 4D-Nucleofector System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection Procedure.
Please email Greg Taborn and Joann Taylor, if you would like to use the 4D-Nucleofector Y Unit.
Location: 3-318
Modular chromatography system for protein purification.
Please email Greg Taborn and Joann Taylor, if you would like to use the Liquid Chromatography System.
When using the centrifuges please sign in via the log sheets general one at the top too
Location: 4-419 LER
- Thermo Sorvall Floor RC 6 Plus Centrifuge
- Rotors in unit or 4-316, Cold Room
- FIBER Lite F-21, 8X50ml
- Sorvall SS-34, 8X50ml
- Sorvall SLA-1500, 6X250ml
- Sorvall SLA-3000, 6X500ml
Location: 4-411
- Beckman Tabletop OPTIMA MAX Ultracentrifuge, 120,000 RPM
Location: 3-419 LER
- Beckman Floor Optima LE 80K Ultracentrifuge
- Beckman Floor Avanti J-26XP Centrifuge
Location: 3-417
- Thermo Sorvall Floor Lynx 4000 Centrifuge (Check with Lucas Turner for approval)
Location: 3-319 LER
- Beckman Floor Optima LE 100K Ultracentrifuge
- Beckman Floor Avanti J-E Centrifuge
Each neighborhood has two (2) PCs and 1 mac available for everyone to use. Printers are also available in each neighborhood.
