Shared Equipment Available at Manne Research Institute
Equipment is available to researchers at Stanley Manne Children’s Research Institute. Trained Lurie Children's users have 24/7 access to the equipment and are given permission to schedule their own sessions.
Guidelines must be followed at all times by those utilizing shared equipment. When using shared equipment, please sign in with the appropriate log sheets. Report any instances of non-compliance or concerns to Joe Dudley, Joann Taylor and Greg Taborn.
The 4D-Nucleofector X Unit supports Nucleofection of various cell numbers (2 x 104 to 2 x 107 ) in different formats.
Please email Greg Taborn and Joann Taylor, if you would like to use the 4D-Nucleofector X Unit.
The Y Unit of the 4D-Nucleofector System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection Procedure.
Please email Greg Taborn and Joann Taylor, if you would like to use the 4D-Nucleofector Y Unit.
Location: 10-510
The QuickPlex instrument offers access to high-performance electrochemiluminescence immunoassays.
Please email Greg Taborn and Joann Taylor, if you would like to use the 1300 MESO QuickPlex SQ120 Imaging System.
Location: 3-426
The ChemiDoc XRS+ Gel Imaging System is a fast detection system for chemiluminescence, fluorescence, and colorimetric imaging.
Please contact Greg Taborn and Joann Taylor if you would like to use the Bio-Rad ChemiDoc XRS+.
Location: 3-509
The Bioruptor Pico allows flexibility in sample volumes (20 µl - 2 ml) and a fast parallel processing of samples (up to 16 samples simultaneously). The built-in cooling system (Bioruptor Cooler) ensures high precision temperature control.
Please email Greg Taborn and Joann Taylor, if you would like to use the Bioruptor Pico sonication devive.
Location: 3-509
The Bioruptor uses a system to uniformly process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications.
Please email Greg Taborn and Joann Taylor, if you would like to use the Bioruptor Plus sonication device.
Location: 4-318
The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. The Chromium Controller enables integrated analysis of single cells at massive scale.
If you are a first-time user requesting training on Chromium Controller 10x Genomics Single Cell please email Greg Taborn and Joann Taylor with an appointment request.
Within the Simpson Querrey Biomedical Research Center, there are various cold storage options, including 4°C, -20°C, -80°C and under-the-bench varieties of equipment. Cold Rooms are also available on each research floor. If you have any questions regarding the use of cold storage equipment, please feel free to contact Joann Taylor or Greg Taborn.
Safeguard the longevity of both the equipment and valuable research samples through consistent maintenance practices. For comprehensive details on maintenance, defrosting procedures and alarm systems specific to freezer equipment, please click here.
Location: 10-308
Droplet Digital™ PCR (ddPCR™) provides ultrasensitive nucleic acid detection and absolute quantification. It is highly effective for resolving low abundance targets, such as allelic or structural variants, that are below the level of detection of other platforms.
The Bio-Rad QX200 ddPCR System including droplet generator and plate reader are available to researchers within Stanley Manne Children’s Research Institute. If you have any questions regarding the use of this shared equipment, please feel free to contact Joann Taylor or Greg Taborn.
Learn more about the Bio-Rad QX200 ddPCR System.
Locations: 4-330 and 3-509
The G:BOX Chemi XX9 gel doc system has highest resolution cameras for imaging multiple sample types and sizes, from fluorescence 1D and 2D gels to chemiluminescent blots
Please email Greg Taborn and Joann Taylor, if you would like to use the IMAGER CHEMILUMINESCENT G-BOX.
Location: 4-509
The LI-COR Odyssey FC imaging system offers superior chemiluminescent detection and the benefits of quantitative infrared wester blot detection and the ability to document DNA gels using the 600nm channel.
Please email Greg Taborn and Joann Taylor if you would like to use the LI-COR Odyssey FC.
Location: 3-318
Modular chromatography system for protein purification.
Please email Greg Taborn and Joann Taylor, if you would like to use the Liquid Chromatography System.
When using the centrifuges, users MUST sign in via the user log located with each centrifuge.
Location: 4-419 LER
- Thermo Sorvall Floor RC 6 Plus Centrifuge
Location: 4-411
- Beckman Tabletop OPTIMA MAX Ultracentrifuge, 120,000 RPM
Location: 3-419 LER
- Beckman Floor Optima LE 80K Ultracentrifuge
- Beckman Floor Avanti J-26XP Centrifuge
Location: 3-319 LER
- Beckman Floor Optima LE 100K Ultracentrifuge
- Beckman Floor Avanti J-E Centrifuge
Each neighborhood has two (2) PCs and 1 mac available for everyone to use. Printers are also available in each neighborhood.
Locations: 4-307, 4-509 and 10-421
The QuantStudio 6 Flex real-time PCR systems can easily interchange between 96-well (0.1 and 0.2 mL) and 384-well formats. The systems can detect differences as small as 1.5-fold in singleplex reactions with 10 logs dynamic range.
Please email Greg Taborn and Joann Taylor, if you would like to use the QUANTSTUDIO 6 FLEX PCR system.
Location: 10-308
Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.
OCR and ECAR rates are key indicators of mitochondrial respiration, glycolysis, and ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
After receiving training from our personnel or Aggilent Representative, users will get access to the room and will be able to reserve the instruments and run their experiments independently.
Send your request for training via email to Greg Taborn and Joann Taylor.
Scheduling for Seahorse XFe96 Analyzer is on a first come, first serve basis. Notification of cancellations is also greatly appreciated.
